Antigen

Antigen Class

TFs and others

Antigen

Tfe3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver tissue

diet intervention

9 days FPC diet

genotype

Control

aav8 injection

GFP (2.5e11 GC)

feeding state when sacrificed

Ad lib

chip antibody

TFE3 (Sigma HPA023881)

chip or input sample

ChIP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Mice were then sacrificed ad lib and livers were harvested. Nuclei lysates were then fixed and sonicated. Chromatin was then saved for input or subject to immunoprecipitation (ChIP) with TFE3 antibody. DNA was then isolated for both input and ChIP samples. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext set 1 and 2 indexes. Sequencing was then done on an Illumina NextSeq 550, via single-read sequencing (75 cycles).

Sequencing Platform

instrument_model

NextSeq 550

mm10

Number of total reads

24794135

Reads aligned (%)

99.0

Duplicates removed (%)

10.5

Number of peaks

7765 (qval < 1E-05)

mm9

Number of total reads

24794135

Reads aligned (%)

98.8

Duplicates removed (%)

10.6

Number of peaks

7892 (qval < 1E-05)